Reporter
GG99

Part:BBa_K2145124:Experience

Designed by: Katie Maruna, Laura Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-18)

Applications of BBa_K2145124

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Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG99 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

Alverno ca 99.png Alverno ca r 99.png Alverno ca g 99.png

Shown above are OD600 measurements, RFP fluorescence, and GFP fluorescence, respectively, for three clones containing this part in liquid culture. Again, we emphasize that only RFP OR GFP are expressed, but never both. We believe this may be due to supercoiling -- when one gene expresses, it creates supercoils that shut down the other gene.

User Reviews

UNIQ92fd13d511a92b90-partinfo-00000000-QINU UNIQ92fd13d511a92b90-partinfo-00000001-QINU